Interactions between Symbiotic Nitrogen Fixation, Combined-N Application, and Photosynthesis in Pisum sativum

The efTect of photosynthetic photon flux density (PPFD) on nitrogen utilization was determined in peas (Pisum sativum L. cv. Alaska) inoculated with Rhizobium leguminosarum and treated with nutrient solutions containing no combined nitrogen, 16 mM NOj", or 16 mM N H / . Plants were grown under controlled conditions at three PPFD values ranging from severely limiting to nearly saturating. Carboxylation efficiencies and COj-exchange rates were highest in the Nj-fixing plants and lowest in plants supplied with NH^+, and they generally increased with increasing PPFD. Photoefficiencies increased with PPFD but did not differ appreciably with the form of nitrogen applied. Nitrogen fixation, calculated from C^Hj-reduction and Hj-evolution data, was inhibited more by N H / than by NO,^ application. Inhibition was counteracted by increasing PPFD. Percentage nitrogen decreased with increasing PPFD in plants treated with combined nitrogen and increased in the plants dependent on N-, fixation. The data reveal that photosynthetic efficiency and the capacity to fix N, in peas are functions of PPFD and the availability of combined nitrogen and that these two factors are interrelated.